Organic & Biomolecular Chemistry · 2014 · 84 citations · 40 references
Natural SciencesBiophysicsChemistryBiophysical ChemistryBiosensorsMolecular ImagingBiophysical SpectroscopyFluorescence ImagingFluorescence MicroscopyFluorescence SpectroscopyMolecular Probe DevelopmentOptical SpectroscopyFluorescence ProbesFluorescent BiosensingFluorescent ProbesFluorescent SensingFret ImagingHigh-sensitivity AssaysRatiometric Fluorescence ImagingSelectivity
We have constructed a new rhodamine-based FRET platform, which was then used to develop a ratiometric fluorescent Cu(2+) probe. The novel Cu(2+) probe exhibits several favorable features including a large variation in the emission ratio, well-resolved emission peaks, high sensitivity, and high selectivity. Importantly, it is suitable for fluorescence imaging in living cells.
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