Structure of a Carbohydrate Moiety of a Unit A Glycopeptide of Calf Thyroglobulin1

Abstract

The structure of the carbohydrate moiety of a unit A glycopeptide of calf thyroglobulin was studied by glycosidase digestion, acetolysis, and methylation analysis. Based on the results, the whole structure of a unit A glycopeptide with the largest carbohydrate moiety was determined as Man alpha 1 leads to 2Man alpha 1 leads to 6(Man alpha 1 leads to 2Man alpha 1 leads to 3)Man alpha 1 leads to 6(Man alpha 1 leads to 2Man alpha 1 leads to 2Man alpha 1 leads to 3)Man beta 1 leads to 4GlcNAc beta 1 leads to 4GlcNAc leads to Asn-peptide.