Detection of a Protein Conformational Equilibrium by Electrospray Ionisation‐Ion Mobility‐Mass Spectrometry

Abstract

Conforming to convention: Evidence for compact and extended forms of cytochrome P450 reductase, believed to exist in dynamic equilibrium in solution, is found in the gas phase by using ion mobility spectrometry. The relative abundance of the two conformations (see picture) can be influenced by the ionic strength of the solution from which they are electrosprayed.