Concepedia

Detection and Toxin Typing of<i>Clostridium perfringens</i>in Formalin-Fixed, Paraffin-Embedded Tissue Samples by PCR

Josephine W. Wu, Wandi Zhang, Boxun Xie, Maoxin Wu, Xiaodi Tong, Jayant Kalpoe, David Zhang

Journal of Clinical Microbiology · 2008 · 54 citations · 15 references

DOI

Concepts

Abstract

Since current microbiology methods are not suitable to detect Clostridium perfringens in formalin-fixed, paraffin-embedded tissue samples, we developed a PCR assay to detect toxin-encoding genes and the 16S rRNA gene of C. perfringens. We successfully detected and genotyped C. perfringens in tissue sections from two autopsy cases.

References

15

Virulence studies on chromosomal α‐toxin and Θ‐toxin mutants constructed by allelic exchange provide genetic evidence for the essential role of α‐toxin in Clostridium perfringens‐mediated gas gangrene

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Molecular typing and epidemiological survey of prevalence of Clostridium perfringens types by multiplex PCR

Han Sang Yoo, S U Lee, K Y Park · Journal of Clinical Microbiology · 1997 · 246 citations · Full text

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Multiplex polymerase chain reaction assay for genotyping Clostridium perfringens.

Meer Rr, Songer Jg · PubMed · 1997 · 232 citations

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Multiplex polymerase chain reaction assay for genotyping Clostridium perfringens

Ralph Meer, J. Glenn Songer · American Journal of Veterinary Research · 1997 · 228 citations · Full text

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Genotyping ofClostridium perfringensby Polymerase Chain Reaction is a Useful Adjunct to Diagnosis of Clostridial Enteric Disease in Animals

J. Glenn Songer · Anaerobe · 1996 · 130 citations

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