Mammalian Cell Cultures for Study of Influenza Virus. II. Virus Propagation.

Abstract

Collagenase-dispersed human and porcine lung cells in primary culture unequivocally propagated human strains A and B, and swine influenza viruses, respectively. Swine lung cells also propagated human influenza A viruses to some extent. Swine virus infection destroyed, and human virus infection less severely damaged porcine and human lung cells respectively. Rabbit lung cells were insusceptible to human and swine influenza viruses. Swine kidney cells propagated egg-infectious swine influenza virus and hemagglutinin without cytopathogenic effect.