Fluorescence‐Based Assay for the Optimization of the Activity of Artificial Transfer Hydrogenase within a Biocompatible Compartment

Abstract

The time capsules: The transfer hydrogenation of an enone-bound fluorogenic compound by an artificial metalloenzyme leads to the release of fluorescent compound umbelliferone. Upon encapsulation of the hybrid catalyst inside a biocompatible compartment, the activity of the transfer hydrogenase is maintained for several months, even at micromolar concentrations. As a service to our authors and readers, this journal provides supporting information supplied by the authors. Such materials are peer reviewed and may be re-organized for online delivery, but are not copy-edited or typeset. Technical support issues arising from supporting information (other than missing files) should be addressed to the authors. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.