Journal of Clinical Pathology · 1994 · 10 citations · 0 references
Life SciencesMicrobiologyMolecular BiologyBacteriologyDiagnostic MicrobiologyMedical MicrobiologyDiagnostic BacteriologyMycobacterial DiagnosticsPathogen DetectionPolymerase Chain ReactionTuberculosis DiagnosisTuberculosis Diagnostic TestingHeat Shock Protein GenesRestriction Fragment AnalysisTuberculosis DiagnosticsTuberculosis Molecular Testing
patients without tuberculosis may also be substantial if the need for treatment trials is reduced.The second issue is the potential of PCR to facilitate efficient laboratory practice.Since our description of the application of PCR to the identification of M tuberculosis in positive Bactec phials, others have devel- oped methods with broader application.Telenti et al identified a large number of species of mycobacteria by restriction diges- tion analysis of a 439 base pair fragment amplified from the gene for the 65 kilodal- ton heat shock protein.'Similarly,