Chronic, multisite, multielectrode recordings in macaque monkeys

TLDR

A paradigm for recording single cortical neuron activity in awake, behaving macaque monkeys is described. The method aims to advance neurophysiological studies of learning, perception, sensorimotor integration, and neuroprosthetic development. It employs high‑density microwire arrays and multichannel instrumentation implanted in up to five cortical areas of three adult rhesus monkeys, with 96–704 wires per subject. Within 3–4 weeks post‑implantation, 421 single neurons were recorded with a mean peak‑to‑peak voltage of 115 ± 3 µV and SNR > 5:1, and up to 247 neurons were captured in a single session, with 58 neurons still isolated 18 months later.

Abstract

A paradigm is described for recording the activity of single cortical neurons from awake, behaving macaque monkeys. Its unique features include high-density microwire arrays and multichannel instrumentation. Three adult rhesus monkeys received microwire array implants, totaling 96–704 microwires per subject, in up to five cortical areas, sometimes bilaterally. Recordings 3–4 weeks after implantation yielded 421 single neurons with a mean peak-to-peak voltage of 115 ± 3 μV and a signal-to-noise ratio of better than 5:1. As many as 247 cortical neurons were recorded in one session, and at least 58 neurons were isolated from one subject 18 months after implantation. This method should benefit neurophysiological investigation of learning, perception, and sensorimotor integration in primates and the development of neuroprosthetic devices.