Characterization and Identification of Streptococci Isolated from Bovine Mammary Glands

Abstract

A total of 317 gram-positive, catalasenegative cocci isolated from bovine mammary glands were characterized and identified using current species descriptions. Two hundred eighty-seven isolates (90.5%) could be placed in 11 distinct species. Streptococcus uberis was the most frequently encountered species and could be separated into two previously described genetic types based upon sucrose utilization. Streptococcus dysgalactiae and a newly described species, Streptococcus saccbarolyticus, were the most frequently isolated organisms from teat canal swabs. Thirty isolates could not be placed in currently described species. A proposed identification scheme based upon serological grouping and seven biochemical tests would permit 24 h identification of streptococci isolated from bovine mammary glands. 17). Current recommended methods (3, 18) utilize the CAMP test, esculin and hippurate hydrolysis, reduction of .1% methylene blue milk, and inulin and raffinose fermentation for differentiation of streptococci isolated from