Identification of a Basement Membrane Zone Antigen Reactive with Circulating IgA Antibody in Ocular Cicatricial Pemphigoid

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

Ulrich K. Laemmli · Nature · 1970 · 251.6K citations

Life Sciences, Microbiology, Molecular Biology +14

Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

Harry Towbin, T. Staehelin, J. Gordon · Proceedings of the National Academy of Sciences · 1979 · 53.8K citations · Full text

Life Sciences, Biochemistry, Molecular Biology +13

Affinity purification of antibodies from diazotized paper blots of heterogeneous protein samples.

J.B. Olmsted · Journal of Biological Chemistry · 1981 · 810 citations · Full text

Life Sciences, Biochemistry, Cell Biology +17

Identification, developmental regulation, and response to heat shock of two antigenically related forms of a major nuclear envelope protein in Drosophila embryos: application of an improved method for affinity purification of antibodies using polypeptides immobilized on nitrocellulose blots

David E. Smith · The Journal of Cell Biology · 1984 · 669 citations · Full text

Life Sciences, Developmental Biology, Molecular Biology +17

Differentiating Anti-Lamina Lucida and Anti-Sublamina Densa Anti-BMZ Antibodies by Indirect Immunofluorescence on 1.0 M Sodium Chloride-Separated Skin

W. Ray Gammon, Robert A. Briggaman, Alfred O. Inman et al. · Journal of Investigative Dermatology · 1984 · 540 citations · Full text

Medicine, Immunology, Pathology +11