BIOCHEMICAL STUDIES ON ADENOVIRUS MULTIPLICATION, XI. EVIDENCE OF A CYTOPLASMIC SITE FOR THE SYNTHESIS OF VIRAL-CODED PROTEINS

Abstract

Available evidence indicates that adenoviruses are assembled in the cell nu- cleus.'-4However, the question of the site of synthesis of viral-specific proteins is not settled.Data reported here provide strong presumptive evidence that adeno- virus-coded proteins, like cellular proteins, are made in the cell cytoplasm on poly- ribosomes.Materials and Methods.-Themethods described by Green and Pifia" 6 were used for cell growth, virus purification, and DNA isolation.Suspension cultures of KB cells at 3 X 105 cells/ml were infected with adenovirus type 2 at an input multi- plicity of 100, and 300-ml amounts were pulsed with 0.5 ,uc/ml of H'-uridine (20 * Paper X in this series is Lacy, S., and M.