Identification of Suitable Reference Genes for qPCR Analysis of Serum microRNA in Gastric Cancer Patients

Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes

Jo Vandesompele, Katleen De Preter, Filip Pattyn et al. · Genome biology · 2002 · 20.1K citations · Full text

Life Sciences, Molecular Biology, Molecular Genetics +14

Circulating microRNAs as stable blood-based markers for cancer detection

Patrick S. Mitchell, Rachael K. Parkin, Evan M. Kroh et al. · Proceedings of the National Academy of Sciences · 2008 · 8K citations · Full text

Medicine, Molecular Biology, Oncology +17

Normalization of Real-Time Quantitative Reverse Transcription-PCR Data: A Model-Based Variance Estimation Approach to Identify Genes Suited for Normalization, Applied to Bladder and Colon Cancer Data Sets

Claus L. Andersen, Jens Ledet Jensen, Torben F. Ørntoft · Cancer Research · 2004 · 7.2K citations

Life Sciences, Biostatistics, Molecular Biology +17

Determination of stable housekeeping genes, differentially regulated target genes and sample integrity: BestKeeper – Excel-based tool using pair-wise correlations

Michael W. Pfaffl, Aleš Tichopád, Christian Prgomet et al. · Biotechnology Letters · 2004 · 5.1K citations

Life Sciences, Genetics, Molecular Biology +12

Real-time quantification of microRNAs by stem-loop RT-PCR

C. Chen · Nucleic Acids Research · 2005 · 5K citations · Full text

Life Sciences, Biotechnology, Molecular Biology +13