Enzyme Electrode for Fructose, Glucose-6-Phosphate and ATP Determination

Abstract

Abstract A bienzyme electrode for glucose-6-phosphate and ATP based on the sequential reactions of coimmobilized glucose-6-phosphate dehydrogenase and hexokinase and for fructose using the competition with glucose has been developed. Electrochemical indication is performed using air-oxidation of reduced N-methylphenazinium ion. The sensor responds linearly to fructose up to 3 mM with a lower detection limit of 0.3 mM.