Removing endotoxin from plasmid samples by Triton X-114 isothermal extraction

Clouding of Triton X-114: The effect of added electrolytes on the cloud point of Triton X-114 in the presence of ionic surfactants

Tingyue Gu, P.A. Galera-Gómez · Colloids and Surfaces A Physicochemical and Engineering Aspects · 1995 · 178 citations

Natural Sciences, Biochemistry, Chemistry +17

Lipopolysaccharide is a frequent contaminant of plasmid DNA preparations and can be toxic to primary human cells in the presence of adenovirus.

Matthew Cotten, A.T. Baker, Mediyha Saltik et al. · PubMed · 1994 · 165 citations

Life Sciences, Microbiology, Molecular Biology +6

Protocol: a rapid and economical procedure for purification of plasmid or plant DNA with diverse applications in plant biology

Jianfeng Li, Li Li, Jen Sheen · Plant Methods · 2010 · 122 citations · Full text

Life Sciences, Biotechnology, Plant Biology +17

Bacterial Lipopolysaccharide Copurifies with Plasmid DNA: Implications for Animal Models and Human Gene Therapy

Ian P. Wicks, Meredith L. Howell, Hitoshi Kohsaka et al. · Human Gene Therapy · 1995 · 87 citations

Life Sciences, Immunology, Molecular Biology +15

Extraction of Superior-Quality Plasmid DNA by a Combination of Modified Alkaline Lysis and Silica Matrix

Lakshmi Ramakrishna, Vijaya Baskar, Udaykumar Ranga · Analytical Biochemistry · 1999 · 26 citations

Life Sciences, Biotechnology, Molecular Biology +13