A Study of Size, Microscopic Morphology, and Dispersion Mechanism of Structures Generated on Hydration of Proliposomes

Abstract

Proliposomes were made by coating lactose monohydrate (LMH), sucrose or sorbitol particles with phosphatidylcholine. Proliposomes hydrated under static (i.e., no shaking) conditions instantly generated liposomes which deaggregated immediately using sorbitol carrier and over a period of 5 minutes using LMH or sucrose particles. Manual dispersion of sucrose-based or sorbitol-based proliposomes generated spherical vesicles, while vesicles generated from LMH-based proliposomes were rich in elongated wormlike structures. The effect of formulation on liposome size was minimal. Overall, carrier type and hydration procedure of proliposomes had a marked effect on liposome morphology while formulation had minimal effect on liposome size.