THE ROLE OF OXYGEN IN THE REGULATION OF ERYTHROPOIESIS. DEPRESSION OF THE RATE OF DELIVERY OF NEW RED CELLS TO THE BLOOD BY HIGH CONCENTRATIONS OF INSPIRED OXYGEN 12

Abstract

Red and white blood cell counts were made on capillary and venous blood with diluting pipettes and hemocytometers standardized by the U. S. Bureau of Standards. Hemoglobin was determined photoelectrically as oxy- hemoglobin (9). Hematocrits and corpuscular constants were obtained by Wintrobe's method (10, 11). Dif- ferential white blood cell counts were done with supravital staining. Dameshek's technic was used for counting reticulocytes and blood platelets (12). Reticulocytes are recorded in terms of number per cu. mm. with percentage levels given in parentheses; each value represents a de- termination on 2,000 to 6,000 red cells, except in Case 8 in which only 1,000 cells were counted routinely.