Loss of sensory neurons after sciatic nerve section in the rat

Abstract

In this study, the loss of sensory neurons in the rat was assessed after sciatic nerve section at birth and at 4 weeks of age. The neuronal deficit in ganglia L4-L6, 39-89 weeks after neonatal denervation, was 10,000-17,000. The nerve contains about 19,000 afferent axons, so some axotomized neurons survived. Degenerating perikarya were absent, probably because all surviving neurons had reestablished target contacts. Sectioning the nerve at age 4 weeks, in five rats, after 19-92 weeks had caused the death of 7,000-11,500 neurons. Whether the nerve regenerated or not in these rats apparently did not influence the extent of neuron death. Nevertheless, no deficit was observed in a sixth rat in which muscle reinnervation was very good. Therefore, the results are inconclusive with respect to the effect of axonal regeneration. Ganglia of rats operated at age 4 weeks regularly contained perikarya with axonal reaction; this supports the notion that some mature neurons are able to permanently survive without target contact. There was no evidence for selective loss of large or small neurons after nerve section at birth or at age 4 weeks. The extent of cell loss in individual ganglia varied, indicating varying contributions of the three ganglia to the nerve. Hence, it is not possible to quantify the effect of experimental conditions on the number of sensory neurons when only one of the several ganglia contributing to the nerve is investigated.