Journal of the American Chemical Society · 2004 · 196 citations · 6 references
Life SciencesBiochemistryMolecular BiologyAnalytical ChemistryBiophysical ChemistryRedox BiochemistryAntioxidant DefenseFluorescence ImagingFluorescence SpectroscopyOxidative Damage To BiomoleculesReactive Oxygen SpeciesRedox SignalingFluorescence-based AssaysFluorescent ProbesFluorescent Reactive Oxygen Species ProbesFluorescent Reporter DesignNadph OxidasesOxidative Stress DetectionRedox-sensitive ProbesSensitivity
Fluorometric detection of O2-* is performed based on desulfonylation of 3 to the corresponding fluoresceins 4 through nucleophilic substitution, and this fluorescing process is quite specific toward O2-* over H2O2, t-BuOOH, NaOCl, 1O2, HO*, NO*, and ONOO-. Furthermore, effects of glutathione, cytochrome P450 reductase/NADPH, and diaphorase/NADH are relatively small on the fluorescing process of probe 3 with X = Y = F, which is useful to detect O2-* released from neutrophils stimulated by phorbol myristate acetate with satisfactory sensitivity.
6