Cloning of a chromosomal locus (exp) which regulates the expression of several exoprotein genes in Staphylococcus aureus

Efficient<i>in vitro</i>synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter

Douglas A. Melton, Paul A. Krieg, Michael R. Rebagliati et al. · Nucleic Acids Research · 1984 · 6.4K citations · Full text

Life Sciences, Biotechnology, Molecular Biology +11

Construction and characterization of new cloning vehicles. II. A multipurpose cloning system. 1977.

Francisco Bolívar, Rodriguez Rl, Greene Pj et al. · PubMed · 1992 · 4.7K citations

Life Sciences, Biotechnology, Genetic Engineering +8

A complementation analysis of the restriction and modification of DNA in Escherichia coli

Herbert W. Boyer, Daisy Roulland-Dussoix · Journal of Molecular Biology · 1969 · 3.9K citations

Life Sciences, Genetics, Microbiology +11

Construction and characterization of new cloning vehicle. II. A multipurpose cloning system

Francisco Bolívar, Raymond L. Rodriguez, Patricia J. Greene et al. · Gene · 1977 · 3.9K citations

Life Sciences, Biotechnology, Genetic Engineering +9

Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene.

Michael Grunstein, David S. Hogness · Proceedings of the National Academy of Sciences · 1975 · 3.5K citations · Full text

Life Sciences, Genetics, Microbiology +17