Directed Evolution of P 450 BM 3 into a <i>p</i>‐Xylene Hydroxylase

Abstract

Reduce, reuse, reengineer! 2,5-Dimethylphenol is of high interest for the production of temperature stable polymers (>500 K) and as a building block for “next-generation” plastics. The presented reengineered P 450 BM 3 variant (R 47 S/Y 51 W/I 401 M) has a good catalytic activity (kcat=1950 min−1), excellent coupling efficiency (66 %), and selectivity (>98 %) for 2,5-dimethylphenol production. Detailed facts of importance to specialist readers are published as ”Supporting Information”. Such documents are peer-reviewed, but not copy-edited or typeset. They are made available as submitted by the authors. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.