Journal of Chemistry · 2008 · 15 citations · 1 references
PharmacyPharmaceutical AnalysisPharmacologyAnalytical ChemistryBiopharmaceuticsChromatographic AnalysisAnalytical ChromatographyBioanalytical Method ValidationChromatographic Analysis Of PharmaceuticalsChromatographic Method ValidationChromatographyLiquid ChromatographyAccuracyAssayChromatographic ValidationDrug Retention TimeHigh-performance Liquid Chromatography Method DevelopmentPrecisionSystem Suitability TestingTablet Chromatographic Analysis
A reverse phase HPLC method is described for the determination of rosiglitazone and gliclazide in tablet dosage form. Chromatography was carried on Phenomenix Gemini C 18 column using in mixture of ammonium phosphate buffer, Acetonitrile and methanol in the ratio 50: 35: 15 v/v as mobile phase at a flow rate 1 mL min -1 and the effluent was monitored at 254 nm. The retention time for rosiglitazone was 3.74 and gliclazide 7.84 min. The limit of detection for rosiglitazone was 4.07μg/mL and gliclazide 1.19 μg/mL. The LOQ obtained for rosiglitazone was 12.33 μg/mL and 3.612 μg/mL. The percentage assay for rosiglitazone was 99.92% and gliclazide was 99.82%. The method was validated for accuracy precision and system suitability. The proposed method was fast accurate and precise so it can be used for regular quality control of the drug.
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