THE CYTOCHEMICAL REDUCTION OF NITRO BLUE TETRAZOLIUM AS AN INDEX OF POLLEN VIABILITY
American Journal of Botany · 1964 · 150 citations · 24 references
BiologyPlant AnalysisBotanyBiochemistryNatural SciencesPlant ReproductionPollen DiagramSymbiosisNitro Blue TetrazoliumNew TechniquePollen Viability
A new technique for estimating pollen viability utilizing Nitro Blue Tetrazolium (Nitro‐BT) is given. Pollen of 16 taxa was studied and scored for stainability. A comparison was made between Nitro‐BT‐stained pollen samples and aniline‐blue‐lactophenol‐stained samples, because the validity of the latter stain has been questioned in recent years. It is demonstrated that Nitro‐BT can discriminate between pollens which have a capacity for oxidative metabolism, representing potential viability, and those which do not. Suggestions for pollen stainability studies are given.
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CYTOCHEMICAL DEMONSTRATION OF SUCCINIC DEHYDROGENASE BY THE USE OF A NEW p-NITROPHENYL SUBSTITUTED DITETRAZOLE
Marvin M. Nachlas, K. C. Tsou, EUSTACE DE SOUZA et al. · Journal of Histochemistry & Cytochemistry · 1957
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THE ESSENTIAL ROLE OF CALCIUM ION IN POLLEN GERMINATION AND POLLEN TUBE GROWTH
J. L. Brewbaker, B. H. Kwack · American Journal of Botany · 1963
1.3K citations
780 citations
George C. Steyskal · Annals of the Entomological Society of America · 1952
487 citations
CALLOSE SUBSTANCE IN PLANT CELLS
H. B. Currier · American Journal of Botany · 1957
379 citations