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An Extracellular Pectate Lyase is the Pathogenicity Factor of the Soft-Rotting Bacterium<i>Pseudomonas viridiflava</i>
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1988
Year
Plant PhysiologyPathogenic MicrobiologyBotanyBacteriologyP. ViridiflavaBacterial PathogensPlant BiologyProkaryotic SystemVirulence FactorAmmonium-sulfate PrecipitationBiologyGram-negative BacteriologyNatural SciencesPathogenicity FactorBiotechnologyPlant Cell CultureMicrobiologyExtracellular Pectate LyaseMedicinePlant Biochemistry
(...) The genetic and biochemical evidence presented here demonstrates that an extracellular pectate lyase (PL) of P. viridiflava is responsible for the maceration of plant tissues. The PL from culture supernatant was purified to apparent homogeneity by ammonium-sulfate precipitation and ion-exchange chromatography (...)