Automation in Microbiology and Immunology

Abstract

Technical methodThe use of this graph paper requires no data processing and is therefore somewhat simpler than the ratio method described by Hales and Randle (1963), as well as more convenient and more accurate than the conventional curve-fitting procedure.Calculation of the total quantities of digoxin bound by the antibody reveals that binding was approximately constant through the range of concentrations tested, as expected from the linearity of the graph shown in the figure.With increasing concentration, however, binding declined slightly, which suggests that it may be inhibited at particularly high concentrations of ligand.The reason for this is unknown at present.