Publication | Open Access
Two-point-separation in super-resolution fluorescence microscope based on up-conversion fluorescence depletion technique
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2003
Year
High ResolutionEngineeringMicroscopyMolecular BiologySuper-resolution Fluorescence MicroscopeBiomedical EngineeringSuper-resolution MicroscopySuper-resolution ImagingMicroscopy MethodNovel Super-resolution MicroscopySpatial ResolutionLight MicroscopyBiophysicsNovel Imaging MethodIndividual Fluorescence SpotsFluorescence ImagingSuper-resolutionFluorescence MicroscopyMicroscope Image ProcessingBiomedical ImagingQuantitative Phase ImagingMedicine
Pronounced separation (750 nm) between two individual fluorescence spots in a novel super-resolution microscopy based on a two-color up-conversion fluorescence depletion technique has been investigated. This microscopy has the potential to achieve a spatial resolution (<300nm) of 1/2 the diffraction limit.