Isolation and Growth of Strains of Xylella fastidiosa from Infected Grapevines on Nutrient Agar Media

Abstract

(...) All media supported growth of both strains, however, the C strain grew more slowly than the FC strain. Four additional strains from grape grew as single colonies on each of the media. The FC and C strains were subcultured weekly on PD3 and nutrient agar-2% sucrose media for 12 mo. The strains remained pathogenic to the grapevine cultivar French Colombard but became less aggressive after 8 mo of subculturing. Colonies of X.f. were reisolated from leaves with symptoms of Pierce's disease