Ultrastructure and Histochemistry of the Food Trapping Mucous Film in Benthic Filter-Feeders (Ascidians)

Abstract

Transmission and scanning electron microscopy of food trapping filters or “mucous films” of several Ascidians reveal the same type of extensive net with elongate rectangular meshes. The ultrastructural appearance and mesh size varies only slightly within the group. Pore sizes from 0.2 to 0.5 μm in width and from 0.5 to 2.2 μm in length with a thickness of the filaments from 10 to 40 nm are probably representative for the nets under in vivo conditions. These observations corroborate earlier experimental results which indicate a remarkably high and constant efficiency of filtration in all species kept undisturbed. Great quantities of water are evidently filtered through these nets at relatively low resistance and particles even below bacterial size (0.5 μm) are effectively trapped. Histochemical reactions indicate the presence of both mucoproteins and acidic polysaccharide components and that the filaments are probably made up of a peptide core surrounded by polysaccharides.