Conversion of Androstenedione to Estrone in Human Fibroblasts Cultured from Prostate, Genital and Nongenital Skin

Abstract

The conversion of (1,2,6,7-3H] androstenedione to [3H]estrone was measured in fibroblast monolayers grown from 3 prostates with benign prostatic hyperplasia (BPH), 3 prostates with prostatic carcinoma, 1 normal prostate of a 26 year old man and from foreskin and nongenital skin sites of 4 males. The reaction product estrone was purified by celite column followed by four successive thin layer chromatographies and final recrystallization to constant 3H/14C ratio.