Applied Physics Letters · 2009 · 42 citations · 16 references
EngineeringMolecular Self-assemblyDna AnalysisMolecular BiologyDna NanotechnologyBiosensing SystemsBioanalysisAnalytical ChemistryBioimagingMolecular ImagingBiophysicsChromatographyDna TargetsDna ReplicationBiomedical AnalysisCholesterol DnaDna DetectionReal-time DetectionBiomolecular ScienceSingle-molecule DetectionBiomolecular EngineeringCrystal/aqueous InterfaceBiomedical DiagnosticsSelf-assemblyMedicine
In this letter, we report a strategy of detecting the DNA targets by using a thin layer of self-assembled cholesterol-labeled DNA probes at the liquid crystal (LC)/aqueous interface. When the system is exposed to 51 μg/ml of complementary DNA targets, the optical appearance of LC shows a continuous change from dark to bright under the crossed polars within 15 min. No obvious change can be observed when the system is exposed to one or two base-pair mismatch DNA targets. This system provides a principle for label-free and real-time detection of DNA targets without any fluorescent labeling.
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Optical Amplification of Ligand-Receptor Binding Using Liquid Crystals
Vinay Gupta, Justin J. Skaife, T. Dubrovsky et al. · Science · 1998 · 468 citations