Temperature fluctuations affect offspring sex but not morphological, behavioral, or immunological traits in the Northern Painted Turtle (Chrysemys picta)

Abstract

Evolutionary theory predicts that when phenotypic variation arises during development that differently influences the fitness of each sex, selection should favor the maternal ability to match offspring phenotype to the sex that incurs a fitness benefit from that phenotype. In reptiles with temperature-dependent sex determination, the temperatures experienced during incubation can influence numerous phenotypic parameters, including sex. To mimic more naturalistic conditions, this experiment examined how variation in temperature fluctuations affects offspring sex as well as a suite of phenotypic parameters having putative fitness consequences in the Northern Painted Turtle ( Chrysemys picta (Schneider, 1783)). We also characterized variation in natural nest temperatures, including the daily temperature range, related to the vegetation cover surrounding the nest. We found that temperature fluctuations did not affect hatchling morphology, immune response, or behavior, but did significantly affect offspring sex ratios. Thermal profiles of natural nests were related to the amount of surrounding vegetation. Results suggest that nest-site choice by females could influence the sex of their offspring, but we found no evidence that variation in temperature fluctuations adaptively matches offspring sex and phenotype.