Analysis of salivary proteins by thin layer sodium dodecylsulphate polyacrylamide gel electrophoresis

Abstract

Samples of the whole, parotid, submandibular and palatine salivas and serum, all collected from the same human subject, were developed by simultaneous electrophoresis on a single SDS thin layer gel plate, and simple proteins, glycoproteins and sialoglycoproteins in the samples were isolated and analysed. Simultaneous electrophoresis of the salivas and serum showed the presence of thirty-four protein bands in serum, twenty-two bands in whole saliva, sixteen bands in parotid saliva, nineteen bands in submandibular saliva and ten bands in palatine saliva. Glycoprotein bands in the isolating gel zone presented five bands in whole saliva, four bands in submandibular saliva, three bands in parotid saliva and no band in palatine saliva. Palatine saliva, however, showed a single clear band in the concentrating gel zone. Palatine saliva showed only one sialoglycoprotein band in the concentrating gel zone.