Tris(pyronato)- and Tris(pyridonato)-ruthenium(III) Complexes and Solution NMR Studies

Abstract

The water-soluble Ru(III) maltolato, ethylmaltolato, and pyridonato complexes, Ru(O-O')3 (O-O' = ma (1a), etma (1b), pyd (2a)), were synthesized (Hma = 3-hydroxy-2-methyl-4-pyrone, Hetma = 2-ethyl-3-hydroxy-4-pyrone, Hpyd = 3-hydroxy-1,2-dimethyl-4-pyridone). The complexes were characterized by elemental analysis, NMR and IR spectroscopies, MS, solution conductivity, and cyclic voltammetry, and in the case of Ru(ma)(3), by X-ray crystallography, which revealed a mer configuration. The paramagnetic 1H NMR resonances of 1a, 1b, and 2a were assigned using 2D methods (1H COSY and 1H-13C HMQC) and variable-temperature 1H NMR data and showed that 1a and 1b exist in aqueous solution predominantly as a mer isomer, while 2a is a mixture of mer and fac isomers. Although a 13C NMR spectrum could not be measured directly for 1a, a partial (13)C spectrum was generated from the 1H-13C HMQC spectrum. Complexes 1a and 1b were tested for anti-proliferatory activity against the human breast cancer cell line MDA-MB-435S and gave IC50 values of 140 and 90 microM, respectively.