Serotonin Binding to Nerve-Ending Particles of the Rat Brain and Its Inhibition by Lysergic Acid Diethylamide

Abstract

The binding of serotonin to nerve-ending particles and other preparations from rat brain has been examined. By investigating the amount bound as a function of serotonin concentration from 10(-7)M to 1O(-2)M, it was possible to identify three major components having K(assoc) (association constant) values of 2 x 10(6), 5 x 10(4), and 5 x 10(2). The component having the highest binding constant was not present in liver and appeared to be confined to the cortex and midbrain regions. This component is inhibited by d-lysergic acid diethylamide at low concentrations. Solubilization of this binding component has been achieved.