Genomic sequence correction by single‐stranded DNA oligonucleotides: role of DNA synthesis and chemical modifications of the oligonucleotide ends

Abstract

We show that, on a chromosomal level, the orientation of the replication fork towards the targeted locus is not central in the strand bias of ssODN-based targeted sequence correction. We demonstrate the importance of accessible ssODN ends for sequence alteration. Finally, we provide evidence for the involvement of DNA synthesis in the process.