Development and characterization of polymorphic microsatellite markers from <i>Fragaria viridis</i>, a wild diploid strawberry

Abstract

Abstract To date, the development of microsatellite (SSR) markers in the genus Fragaria has focused on F. vesca . However, further species are thought to have contributed to the complex allo‐octoploid genome of the cultivated strawberry, F. × ananassa . Here, we present 22 new SSR markers developed from the diploid species F. viridis . Twenty‐one of the primer pairs amplified polymorphisms in six F. viridis accessions, with an average of 4.95 alleles per primer pair and an average expected heterozygosity of 0.68. Fourteen of these primer pairs, and a locus monomorphic in F. viridis , amplified polymorphic alleles in the parents of a F. vesca mapping population.