A Jar Fermentor Culture of Nicotiana tabacum L. Cell Suspensions

Abstract

A Jar fermentor technique used for microbes is applied to a large-scale culture of tobacco cell suspension in both batch and semi-continuous culture systems. A 30 liter fermentor contained 20 liters culture which, after 5 days incubation at 28°C, yielded a suspension of 20g/liter as dry weight and 650ml/liter as packed cell volume in a batch system. Growth curves of tobacco cell suspensions showed an optimal mean doubling time of 1 day during an exponential growth phase of 1_??_4 days. In the semi-continuous cultures, the yields of dry weight were 12_??_13g/liter day.