Characterization of Mutations in the Beta-Tubulin Gene of Benomyl-Resistant Field Strains of<i>Venturia inaequalis</i>and Other Plant Pathogenic Fungi

Abstract

All benomyl-resistant field strains of Venturia inaequalis. V. pirina, Monilinia fructicola, Sclerotinia homoeocarpa, and six species of Penicillium, except those with low resistance to benomyl, were found to contain a single base pair mutation in their beta-tubulin gene that resulted in an amino acid substitution in beta-tubulin. In V. inaequalis, codon 198, which encodes glutamic acid in a sensitive strain, was converted to a codon for alanine in a strain with very high resistance, to a codon for lysine in a strain with high resistance, or to a codon for glycine in a strain with medium resistance to benomyl (...)