Study of<i>Saccharomyces cerevisiae</i>Yeast Cells by Field-Flow Fractionation and Image Analysis

Abstract

Abstract Asymmetrical flow field-flow fractionation has been applied to characterize Sac-vliaronivces cvrcvisiiw cells, the morphology of which has been assessed by image analysis during fermentation on glucose medium. Intact cells, budding or nonbud-ding, are eluted and separated according to the steric-hyperlayer modes, while macromolecules and other cell debris are eluted according to the normal mode.