The identification of the potato cyst nematodes Globodera rostochiensis and G. pallida by isoelectric focusing of proteins on polyacrylamide gels

Abstract

SUMMARY Isoelectric focusing on thin layers of polyacrylamide gel was used to separate proteins from the potato cyst‐nematodes Globodera rostochiensis and G. pallida. General protein patterns could be used to identify the two species, even from. single cysts. Staining for the enzyme phosphoglucomutase (PGM) also produced species specific patterns and the presence of PGM variation among G. pallida populations was revealed. The potential of isolectric focusing as a routine research and advisory tool in nematology is discussed.