Multilocus Sequence Typing of <i>Klebsiella pneumoniae</i> Nosocomial Isolates

TLDR

The study sequenced seven housekeeping genes from 67 Klebsiella pneumoniae strains, including 19 ceftazidime‑ and ciprofloxacin‑resistant isolates. The developed MLST scheme identified 40 distinct allelic profiles, was validated against ribotyping with 96 % discriminatory power, and provides unambiguous data for K.

Abstract

ABSTRACT A multilocus sequence typing (MLST) scheme was developed for Klebsiella pneumoniae . Sequences of seven housekeeping genes were obtained for 67 K. pneumoniae strains, including 19 ceftazidime- and ciprofloxacin-resistant isolates. Forty distinct allelic profiles were identified. MLST data were validated against ribotyping and showed high (96%) discriminatory power. The MLST approach provides unambiguous data useful for the epidemiology of K. pneumoniae isolates.