A NEW ANTI‐INFLAMMATORY TEST, UTILIZING THE CHORIO‐ALLANTOIC MEMBRANE OF THE CHICK EMBRYO

Abstract

Existing methods for the detection of anti-inflammatory activity involve the use of experimental animals. These methods depend on the production of an experimental inflammation in the animal and the reduction of this inflammation by repeated doses of an anti-inflammatory agent. Such methods in common use include " the formalin foot test" (Selye, 1949), "the cotton pellet test" (Meier, Schuler & Desaulles, 1950), "the B.C.G. test " (Long & Miles, 1950), "the ultra-violet erythema test " (Wilhelmi & Domenjoz, 1951), " the granuloma pouch test " (Selye, 1953), " the pleural exudate test " (Holtkamp, Wang & Doggett, 1958), and various modifications of these methods (Buttle, D 'Arcy, Howard & Kellett, 1957; In addition, various capillary permeability tests utilizing the spread of dyes through tissue have been introduced (Bacharach,