Diversity of Properties among Satsuma Dwarf Virus and Related Viruses.

Abstract

Serological property and electrophoretic mobility of coat protein as well as symptoms on citrus and herbaceous plants were compared on two isolates (S-58, MIE-88) of satsuma dwarf virus (SDV) and four of related viruses (Ci-968, LB-1, Az-1, NI-1), using enzyme-linked immunosorbent assay (DAS-ELISA), sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and electro-blot immunoassay. Symptoms on citrus and herbaceous plants showed that S-58 and MIE-88 are SDV, Ci-968 is citrus mosaic virus (CiMV), NI-1 is navel orange infectious mottling virus (NIMV), while LB-1 and Az-1 are unreported viruses. In DAS-ELISA, S-58 and MIE-88 reacted strongly to anti-S-58 polyclonal antibody (S-PA), Ci-968 and LB-1 strongly to anti-Ci-968 antibody (Ci-PA) while Az-1 and NI-1 reacted to neither of them. SDS-PAGE revealed that S-58 and MIE-88 had two major coat proteins of about 42, 000 and 22, 000 daltons (slow migrating band, SB and fast migrating band, FB), Ci-968, LB-1 and Az-1 had ones of about 42, 000 and 23, 000 daltons, and NI-1 had ones of about 42, 000 and 22, 500 daltons. In electro-blot immunoassay, S-PA reacted strongly to SB of S-58 and MIE-88, Ci-PA reacted strongly to SB of Ci-968, LB-1 and Az-1, and relatively weakly to SB of S-58 and MIE-88, while neither of S-PA and Ci-PA reacted to SB of NI-1. FB of S-58 and MIE-88 reacted very weakly to S-PA and Ci-PA. FB of Ci-968, LB-1, Az-1 and NI-1 reacted to neither of S-PA and Ci-PA. These results show that the six isolates can be classified as follows; SDV group (S-58 and MIE-88), CiMV group (Ci-968, LB-1 and Az-1) and NIMV group (NI-1). However, it was considered that more data should be accumulated to define these groups as distinct viruses or strains of SDV.