Free amino acids in the synaptosome and synaptic vesicle fractions of different bovine brain areas

Abstract

Free amino acids were quantitatively estimated in intact tissues and isolated synaptosome and synaptic vesicle fractions of the bovine brain regions with the aid of a sensitive amino acid analyzer. The brain areas studied were frontal, parietal and occipital cerebral cortices, cerebellar cortex, caudate and lenticular nuclei, superior colliculi, thalamus, pons and medulla. The most abundant amino acid in tissue samples and synaptosome fractions was glutamic acid, followed by glutamine, aspartic acid, GABA and taurine. The dominatating amino acid in all isolated synaptic vesicle fractions was taurine. The concentrations of glutamic acid, glutamine, GABA and aspartic acid were generally much lower. The 5 transmitter candidates, viz. GABA, glycine, glutamic acid, as particaid and taurine, comprised about one-half of the total amino acids in all samples. Taurine was the only amino acid highly enriched in the vesicle fractions. This enrichment was discernible in all brain areas. It is suggested therefore that taurine is rather a ubiquitous associate of synaptic membrane structures than a specific inhibitory transmitter.