AIDS Research and Human Retroviruses · 1989 · 64 citations · 22 references
Life SciencesInfectious DiseasesMolecular BiologyMolecular VirologyStructural BiologyVirologyViral InfectionsViral PathologyViral ReplicationViral StructureViral TropismVirus StructureAmino AcidsTransmembrane DomainsTransmembrane ProteinsViral Envelope GlycoproteinsViral Envelope ProteinsViral GlycoproteinsViral Protein ConformationViral Replication Kinetics
The transmembrane glycoprotein (gp41) of human immunodeficiency virus type-1 (HIV-1) has a long cytoplasmic domain of unknown functional significance. To investigate the role of the carboxy-terminal (C-terminal) portion of the HIV-1 envelope protein in viral replication, infectivity, and cytopathogenicity, we examined the properties of a panel of mutants with variable deletions in the 3'-env region. Deletion of the C-terminal 76 amino acids did not abolish production of reverse transcriptase upon transfection of COS-1 cells. Deletion of the C-terminal 6-14 amino acids appeared sufficient to alter the replication pattern, infectivity, and cytopathogenicity of some clones. The data suggest that conformational determinants or specific sequences are responsible for the observed changes, rather than simply the length of the gp41 cytoplasmic tail.
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Bernard J. Poiesz, Francis W. Ruscetti, Adi F. Gazdar et al. · Proceedings of the National Academy of Sciences · 1980 · 5.1K citations · Full text
Functional Regions of the Envelope Glycoprotein of Human Immunodeficiency Virus Type 1
Mark Kowalski, Joseph Potz, Ladan Basiripour et al. · Science · 1987 · 849 citations