Electrochemical Oxidation of 8-Oxoguanine

Abstract

Electrochemical oxidation of DNA can occur at each of the four bases and guanine is the one that can suffer the easiest oxidative damage. The occurrence of the guanine oxidation product, 8-oxoguanine, as a consequence of DNA damage caused by DNA oxidation causes important mutagenic lesions and hence it is very important to develop reliable methods for its quantification. Electrochemical study of the mechanism of oxidation of 8-oxoguanine on glassy carbon shows that it is a reversible electrode process, pH dependent, and involves several reaction products. Electroanalytical determinations of 8-oxoguanine were carried out and the detection limit was 8×10–7 M.