Concepedia

Staining Tissue for Light and Electron Microscopy by Bridging Metals with Multidentate Ligands

Jacob S. Hanker, Chandicharan Deb, Hannah L. Wasserkrug, Arnold M. Seligman

Science · 1966 · 63 citations · 9 references

DOI

Concepts

Abstract

"Osmium black," a pigment very useful for cytological staining in both light and electron microscopy, may be deposited selectively at the tissue-binding sites of other metal ions by bridging OsO(4) to the tissue bound metal ion through a multidentate ligand.

References

9

A NEW STAINING METHOD (OTO) FOR ENHANCING CONTRAST OF LIPID-CONTAINING MEMBRANES AND DROPLETS IN OSMIUM TETROXIDE-FIXED TISSUE WITH OSMIOPHILIC THIOCARBOHYDRAZIDE (TCH)

Arnold M. Seligman, Hannah L. Wasserkrug, Jacob S. Hanker · The Journal of Cell Biology · 1966 · 347 citations · Full text

, , +11

Improvement in the Histochemical Localization of Leucine Aminopeptidase with a New Substrate, <i>L</i>-Leucyl-4-Methoxy-2-Naphthylamide

Marvin M. Nachlas, Benito Monis, David S. Rosenblatt et al. · The Journal of Cell Biology · 1960 · 318 citations · Full text

, , +13

Stability Constants of Internal Complexes

David Paver Mellor, LE Maley · Nature · 1947 · 174 citations

, , +11

HISTOCHEMICAL DEMONSTRATION OF SOME OXIDIZED MACROMOLECULES WITH THIOCARBOHYDRAZIDE (TCH) OR THIOSEMICARBAZIDE (TSC) AND OSMIUM TETROXIDE

Arnold M. Seligman, Jacob S. Hanker, Hannah L. Wasserkrug et al. · Journal of Histochemistry & Cytochemistry · 1965 · 174 citations

, , +9

Osmiophilic Reagents: New Cytochemical Principle for Light and Electron Microscopy

Jacob S. Hanker, Arlene R. Seaman, Leon Weiss et al. · Science · 1964 · 168 citations

, , +8