Biotechnology Progress · 1996 · 179 citations · 16 references
DentistryDental Biomaterials ScienceOral BiologyOral Tissue RegenerationRoot Canal TherapyTissue EngineeringBiomaterials For Oral RegenerationDental BiomaterialsDental PulpPulp Cell BiologyPulp PhysiologyPulp RepairBiocompatibility Of Regenerative MaterialsPolyglycolic AcidPulp FibroblastsPulp Tissue RepairPulpal Tissue RegenerationSynthetic Extracellular MatrixSynthetic Substitute MaterialsTooth Tissue Engineering
Injury or infection of adult dental pulp often requires root canal therapy, halting dentin formation and tooth maturation, and current synthetic replacements cannot fully restore lost tissue function and tend to fail over time. The study reports a technique to engineer new pulp‑like tissues using cultured cells and synthetic extracellular matrices. Fibroblasts isolated from adult dental pulps were expanded in culture and seeded onto polyglycolic acid fiber matrices to create the engineered tissue. The engineered fibroblasts adhered to the fibers, proliferated, and formed a 60‑day tissue with cellularity comparable to native pulp, suggesting potential use in oral tissue regeneration and as a platform for testing dental materials.
Abstract Injury or infection of adult dental pulp often necessitates root canal therapy. This terminates dentin formation and subsequent tooth maturation. In addition, the synthetic materials currently utilized to replace lost tooth structure are not capable of completely replacing the function of the lost tissue, and often fail over time. This report describes a technique to engineer new pulp‐like tissues utilizing cultured cells and synthetic extracellular matrices. Fibroblasts were obtained from human adult dental pulps and multiplied in culture. These cells were subsequently seeded onto synthetic matrices fabricated from fibers (approximately 15 μm in diameter) of polyglycolic acid (PGA). The pulp‐derived fibroblasts adhered to the fibers, proliferated, and formed a new tissue over 60 days in culture with a cellularity similar to that of native pulp. These tissues may find application in the regeneration of oral tissues and may provide novel systems in which to study the biocompatibility of materials and chemicals used in dentistry.
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Fluorometric assay of DNA in cartilage explants using Hoechst 33258
Young Jo Kim, Robert L. Sah, Joe‐Yuan H. Doong et al. · Analytical Biochemistry · 1988 · 1.4K citations
J.A. Salzmann · American Journal of Orthodontics · 1977 · 614 citations