Endpoint quantitative PCR assays for <i>Bacteroides forsythus</i>, <i>Porphyromonas gingivalis</i>, and <i>Actinobacillus actinomycetemcomitans</i>

Abstract

Amplifluor qPCR assays discriminated between plaque samples differing by one log or more, allowing major infections to be distinguished from minor ones. This approach allows high-throughput qPCR of plaque samples, using equipment available to many laboratories.