Topology of eukaryotic type II membrane proteins: Importance of N-terminal positively charged residues flanking the hydrophobic domain

DNA sequencing with chain-terminating inhibitors

Frederick Sanger, S. Nicklen, Alan Coulson · Proceedings of the National Academy of Sciences · 1977 · 69.1K citations · Full text

Dna, Engineering, Dna Analysis +20

Transfer of proteins across membranes. I. Presence of proteolytically processed and unprocessed nascent immunoglobulin light chains on membrane-bound ribosomes of murine myeloma.

Günter Blobel, B Dobberstein · The Journal of Cell Biology · 1975 · 3K citations · Full text

Protein Secretion, Murine Myeloma, Immunology +18

Eukaryotic transient-expression system based on recombinant vaccinia virus that synthesizes bacteriophage T7 RNA polymerase.

Thomas R. Fuerst, Edward G. Niles, F. William Studier et al. · Proceedings of the National Academy of Sciences · 1986 · 2.1K citations · Full text

Vaccination, Viral Replication, Molecular Virology +15

Intracellular protein topogenesis

Günter Blobel · Proceedings of the National Academy of Sciences · 1980 · 1.4K citations · Full text

Translocation Process, Stop-transfer Sequences, Molecular Biology +22

The spontaneous insertion of proteins into and across membranes: The helical hairpin hypothesis

Donald M. Engelman, Thomas A. Steitz · Cell · 1981 · 873 citations

Proteinlipid Interaction, Protein Secretion, Protein Assembly +13