Short communication: Erythrocyte binding antigen (EBA‐175) of <i>Plasmodium falciparum</i>: improved genotype determination by nested polymerase chain reaction

Abstract

We have designed primers to the conserved region of the erythrocyte binding antigen (EBA)-175 gene which amplify specifically the two alleles by polymerase chain reaction (PCR) and by nested PCR. This approach provides a specific, sensitive and rapid method for genotype determination in a large number of Plasmodium falciparum field isolates.